Description
Develosil 100 Diol / 300 Diol
Develosil 100Diol and 300Diol are the columns developed for gel filtration chromatography.
The size exclusion chromatography of water soluble polymers, such as hundreds of thousands of protein,
enzymes, etc., is possible from the molecular weight 10,000.
packing material type: For the compounds of low molecular weight ⇒ 100 Diol
For the amounts of macromolecules ⇒ 300 Diol
That is, since there are two kinds, low molecular weight and the amount of macromolecules, a column
can be chosen according to the molecular weight of the purpose reagent.
Develosil Diol Series Specifications
| Column Name | Ligand | Carbon | End Capping | Surface Area | Pore Diameter | Pore Volume | Range of pH |
|---|---|---|---|---|---|---|---|
| 100 Diol | Glycero propyl radical |
12% | No | 350 m²/g | 120 Å | 1.05 ml/g | pH 2-7.5 |
| 300 Diol | 9% | 180 m²/g | 250 Å |
Size Exclusion Limit for Develosil Diol Series
| Column Name | Proteinic Exclusion Limit Molecualr Weight | Polyethylene Glycol Exclusion Limit Molecular Weight |
|---|---|---|
| 100 Diol | 100,000 | 10,000 |
| 300 Diol | 1,000,000 | 100,000 |
The Develosil 100 Diol and 300 Diol was designed for gel filtration using Develosil’s high quality Si gel. This column is used worldwide in food research specifically with proanthocyanidins.
Other typical uses for this column is size exclusion chromatography of large water-soluble compounds such as proteins and enzymes.
For low molecular compounds, we recommend the 100 Diol; and for large macromolecular compounds, we recommend the 300 Diol.
Featured Applications
Identification of Flavo-3-ols in Avocado using HPLC-FLD
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil 100 Diol 5 µm (250 x 4.6 mm) |
| Mobile Phase: | A. Acidic Acetonitrile (ACN:Acetic Acid, 98:2, v/v) B. Acidic aqueous methanol (MeOH:Water:Acetic Acid, 95:3:2, v/v) |
| Gradient: | 1. 7% B for 3 min 2. 37.6% B for 57 min. 3. 100% B for 7 min. 4. 7% B for 5 min. |
| Detection: | Fluorescence Detector (Excitation wavelength-230 nm; Emission wavelength 321 nm) |
| Sample: | Various Degrees of Polymerization of Flavo-3-ols in: 1. Avocado Pulp 2. Avocado Skin 3. Avocado Seed |
| Injection Volume: | 5.0 µL |
Ana López-Cobo, Ana María Gómez-Caravaca, Federica Pasini, María Fiorenza Caboni, Antonio Segura-Carretero, Alberto Fernández-Gutiérrez, HPLC-DAD-ESI-QTOF-MS and HPLC-FLD-MS as valuable tools for the determination of phenolic and other polar compounds in the edible part and by-products of avocado, LWT, Volume 73, 2016, Pages 505-513, ISSN 0023-6438, https://doi.org/10.1016/j.lwt.2016.06.049.
Analysis of Procyanidins in Cocoa using HILIC-FLD
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil 100 Diol, 5 µm (250 mm x 1mm) |
| Mobile Phase: | A. Acidic Acetonitrile (ACN:Acetic Acid, 99:1, v/v) B. Acidic aqueous methanol (MeOH:Water:Acetic Acid, 94.05:4.95:1, v/v) |
| Detection: | Fluorescence Detector (Excitation wavelength-230 nm; Emission wavelength 321 nm) |
| Sample: | Various Degrees of Polymerization of procyandin oligomers in cocoa extract |
| Other analytical conditions can be found in article. | |
