Description
DEVELOSIL ANIDIUS
The Develosil ANIDIUS was specifically designed for HILIC operations. The bonded phase is comprised of -OH and -NH2 groups and is bonded onto the silica base using a proprietary technology. The Develosil ANIDIUS is most suitable for high polarity compounds that cannot be separated using reverse phase chromatography.
Develosil ANIDIUS Specifications
| Bonded Phase | Carbon | End Capping | Surface Area | Pore Diameter | Pore Volume | Range of pH | Particle Size |
|---|---|---|---|---|---|---|---|
| -OH and -NH2 | 8% | Yes | 300 m²/g | 140 Å | 1.15 ml/g | pH 2-7.5 | 5 µm |
Featured Applications
Analysis of Saccharides
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil ANIDIUS (4.6 x 250 mm) |
| Mobile Phase: | Acetonitrile/Water=80/20 |
| Flow Rate: | 1.0 mL/min |
| Detection: | RI |
| Sample: | Fructose, Glucose, Sucrose, Maltose |
| Injection Volume: | 20 µL |
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil ANIDIUS (4.6 x 250 mm) |
| Mobile Phase: | Acetonitrile/Water=65/35 |
| Flow Rate: | 1.0 mL/min |
| Detection: | RI |
| Sample: | Glucose, maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose |
| Injection Volume: | 20 µL |
Analysis of Water-soluble Vitamins
HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil ANIDIUS (4.6 x 250 mm) |
| Mobile Phase: | A) Acetonitrile + 0.1% Formic Acid B) 0.1% Formic Acid |
| Gradient: | B) 25-60% (15 minutes) -> 60% (30mins) |
| Flow Rate: | 0.5 mL/min |
| Detection: | UV at 280 nm |
| Sample: | 1. Thiamine (0.5 mg/mL) 2. Nicotineamide (1.0 mg/mL) 3. Riboflavin (1.0 mg/mL) 4. Cyancobalamin (1.0 mg/mL) 5. Nicotinic Acid (1.0 mg/mL) 6. Ascorbic acid (3.0 mg/mL) |
| Injection Volume: | 2.0 µL |
