Description
Benson HPLC is currently the most important chromatographic method for analyzing carbohydrates, alcohols and organic acids. This technique is easy to use, yet capable of rapid, specific, sensitive and precise measurements. We at Benson Polymeric understand that choosing a column for HPLC analysis can be a difficult decision based on your unique sample, therefore we take the steps necessary to guarantee a column will meet your specific needs. Our company offers several types of polymeric based columns for the analysis of carbohydrates, organic acids, and alcohols. Please contact us in regards to your specific separation needs. We will help you choose a replacement column for your application, or recommend a column for a new assay.
Benson Polymeric offers a wide variety of columns for the analysis of carbohydrates. All of our carbohydrate columns are packed with polymeric materials, Sulfonated Poly(Styrene-Divinylbenzene), specifically designed to maximize your separation needs. Benson Polymeric columns utilize a variety of separation mechanisms that allow carbohydrates to be separated without the need of gradients. By altering the ionic form of our polymers (calcium, lead, sodium, silver, and potassium) specific carbohydrate mixtures can be separated (see Applications section) by simply using water as your mobile phase.
Another technique Benson Polymeric uses to maximize your separation is to offer a range of cross-linked polymers (see Polymer Description). The degree of cross-linkage determines the porosity of the polymers which can be used to enhance certain separations. Benson Polymeric recommends using column ovens in combination with our columns since the best separations are usually obtained at elevated temperatures (typical range 30 – 90 Celsius).
Benson’s sole focus is to provide high quality polymeric products. We are able to offer a complete line of columns at competitive pricing. Not only can we reduce your analysis costs, we also provide quick and knowledgeable service to our customers.
BP-100 H USP L17, Wines, Dairy, Bio-Reactions, Medical Science
BP-800 H USP L17, Wines, Dairy, Bio-Reactions, Medical Science
BP-100 Ca USP L19, High fructose corn syrup, sugar alcohols, sugars, USP analysis of mannitol and sorbitol
BP-200 Ca USP L19, High fructose corn syrup, sugar alcohols, sugars
BP-800 Ca USP L19, Sweetners, monosaccharides, high fructose corn syrup, di-, tri, and tetrasaccharides, sugar alcohols, mannitol and sorbitol
BP-200 Na Oligosaccharide analysis up to DP11 in samples containing salt
BP-800 Na Molasses and other sugars in high salt sample
BP-100 Ag Specific for Oligosaccharides in beer and corn syrup
BP-200 Ag Oligosaccharide analysis up to DP11
BP-100 Pb USP L34, Biomass derived sugar samples, lactose, sucrose, maltose
BP-800 Pb USP L34, Cellulose-derived monosaccharides, pentoses and hexoses from wood products, dairy products (sucrose, lactose, fructose)
BP-100 K Mono-, di-, trisaccharide analysis in corn syrup and brewing wort samples, glucose, maltose, maltotriose, betaine.
BP-800 K Mono-, di-, trisaccharide analysis in corn syrup and brewing wort samples, glucose, maltose, maltotriose, betaine.
BP-800 H USP analysis of citric, lactic and acetic acid
BP-800 H Rapid screening of fruit samples such as grape must, ethanol, acetic acid, glycerol, fructose, glucose
BP-800 H Dairy products, food additives, flavor indicators, food stability, vitamin content, ascorbic acid, and nutritional analysis
BP-100 H Dairy products, food additives, flavor indicators, food stability, vitamin content, ascorbic acid, and nutritional analysis
Column Comparison
Benson Polymeric offers a complete line of high quality and cost effective columns for analysis of organic acids. The chart below lists some of our recommended replacement columns for polymeric columns offered by other suppliers. For specific recommendations on the column and method best suited to maximize the separation of your particular sample please do not hesitate to contact the support staff at Benson Polymeric.
| Benson Column Description | Benson Part # | Bio-Rad Part # (Aminex) | Phenomenex Part # (Rezex) | Varian Part # (Metacarb) | Dionex Part # | Transgenomic Part # | Waters / Hamilton Part # | Shodex Part # | Grace / Alltech Part # |
|---|---|---|---|---|---|---|---|---|---|
| BP-800 H | 8112-0 | 064198 | ICE-99-8461 | 79476 | |||||
| BP-800 H | 8111-0 | 125-0100 | 00D-0223-KO | A5059 | ICE-99-5861 | 269068 | |||
| BP-800H | 8100-0 | 125-0140 | 00H-0132-KO 00H-3252-KO |
A5210 | 043197 | ICE-99-9861 ICE-99-9810 |
79544 | F6378100 F6378030 |
269006 |
| BP-100 H | 1100-0 | A5215 | ICE-99-9850 | 9646 | |||||
| BP-100 H Guard | 1100-2 | A5211 A5216 |
067842 | CHO-99-3561 | |||||
| BP-800 H Guard | 8100-2 | A5211 A5216 |
067842 | CHO-99-3561 |
| Benson Column Description |
Benson Part # |
Column Size |
Bio-Rad Part # (Aminex) |
Phenomenex Part # (Resex) |
Agilent Part # |
Varian Part # |
Concise Separations Part # |
Shodex Part # |
Miscellaneous Part # |
|---|---|---|---|---|---|---|---|---|---|
| BP-100 Ca | 1000-0 | 300 x 7.8 mm | |||||||
| BP-100 Ca | 1070-0 | 300 x 6.5 mm | PL1F70-6850 | CHO-99-9753 | Waters WAT085188 Alltech 70057 |
||||
| BP-200 Ca | 1500-0 | 300 x 7.8 mm | 125-0096 | Hamilton 79432 | |||||
| BP-200 Na | 1550-0 | 300 x 7.8 mm | 00P-0137-NO | PL1171-6140 | A5238 | CHO-99-9850 | |||
| BP-200 Ag | 1600-0 | 300 x 7.8 mm | 125-0097 | 00P-0133-NO | A5223 | CHO-99-9851 | |||
| BP-100 Pb | 1200-0 | 300 x 7.8 mm | A5220 | CHO-99-9854 | |||||
| BP-800 Ca | 8000-0 | 300 x 7.8 mm | 125-0095 | 00H-0130-KO | PL1170-6810 | A5200 A5205 |
CHO-99-9860 CHO-99-9855 |
F6378102 | Hamilton 79436 |
| BP-800 Ca | 8040-0 | 250x 4.0 mm | 125-0094 | PL1570-5810 | A5092 | CHO-99-8453 | MN-431 | Hamilton 79431 | |
| BP-800 K | 8300-0 | 300 x 7.8 mm | 125-0142 | 00H-3252-KO | PL1170-6860 | A5095 | CHO-99-9862 | ||
| BP-800 H | 8100-0 | 300 x 7.8 mm | PL1170-6830 | ||||||
| BP-800 Na | 8700-0 | 300 x 7.8 mm | 125-0143 | 00H-0136-KO | PL1170-6840 | A5041 | CHO-99-9863 | F6378010 | |
| BP-800 Pb | 8200-0 | 300 x 7.8 mm | 125-0098 | 00H-0135-KO | PL1170-6820 | A5241 | CHO-99-9864 | F6378105 | Hamilton 79476 |
| Benson Polymer Description | Benson Part # | Cross-Linkage | Particle Size | Cationic Form | MW Exclusion Limit | Wet Capacity (meq/ml) | Normal Density (g/ml) |
|---|---|---|---|---|---|---|---|
| BP-100 Ca | 4100-0 | 6 | 9 | Calcium | 1200 | 1.4 | 0.8 |
| BP-800 Ca | 4105-0 | 8 | 9 | Calcium | 1000 | 1.7 | 0.8 |
| BP-200 Ca | 4200-0 | 4 | 16 | Calcium | 1400 | 1.1 | 0.8 |
| BP-100 Na | 4120-0 | 6 | 9 | Sodium | 1200 | 1.4 | 0.8 |
| BP-800 Na | 4125-0 | 8 | 9 | Sodium | 1000 | 1.7 | 0.8 |
| BP-200 Na | 4210-0 | 4 | 16 | Sodium | 1400 | 1.1 | 0.8 |
| BP-100 Pb | 4130-0 | 6 | 9 | Lead | 1200 | 1.4 | 0.8 |
| BP-800 Pb | 4135-0 | 8 | 9 | Lead | 1000 | 1.7 | 0.8 |
| BP-100 Ag | 4140-0 | 6 | 9 | Silver | 1200 | 1.4 | 0.8 |
| BP-200 Ag | 4240-0 | 4 | 16 | Silver | 1400 | 1.1 | 0.8 |
| BP-100 K | 4150-0 | 6 | 9 | Potassium | 1200 | 1.4 | 0.8 |
| BP-800 K | 4155-0 | 8 | 9 | Potassium | 1200 | 1.7 | 0.8 |
| Benson Polymer Description | Benson Part # | Cross-Linkage | Particle Size | Cationic Form | MW Exclusion Limit | Wet Capacity (meq/ml) | Normal Density (g/ml) |
|---|---|---|---|---|---|---|---|
| BP-100 H | 4110-0 | 6 | 9 | Hydrogen | 1200 | 1.4 | 0.8 |
| BP-800 H | 4310-0 | 8 | 9 | Hydrogen | 1000 | 1.7 | 0.8 |
Carbohydrate Compounds
A carbohydrate is a biological molecule consisting of carbon (C), hydrogen (H) and oxygen (O) atoms, usually with a hydrogen–oxygen atom ratio of 2:1 (as in water); in other words, with the empirical formula Cm(H2O)n (where m could be different from n). This formula holds true for monosaccharides. Some exceptions exist; for example, deoxyribose, a sugar component of DNA, has the empirical formula C5H10O4. Carbohydrates are technically hydrates of carbon; structurally it is more accurate to view them as polyhydroxy aldehydes and ketones.
The term is most common in biochemistry, where it is a synonym of ‘saccharide’, a group that includes sugars, starch, and cellulose. The saccharides are divided into four chemical groups: monosaccharides, disaccharides, oligosaccharides, and polysaccharides. Monosaccharides and disaccharides, the smallest (lower molecular weight) carbohydrates, are commonly referred to as sugars. The word saccharide comes from the Greek word σάκχαρον (sákkharon), meaning “sugar”. While the scientific nomenclature of carbohydrates is complex, the names of the monosaccharides and disaccharides very often end in the suffix -ose. For example, grape sugar is the monosaccharide glucose, cane sugar is the disaccharide sucrose, and milk sugar is the disaccharide lactose.
Organic Acid Compounds
An organic acid is an organic compound with acidic properties. The most common organic acids are the carboxylic acids, whose acidity is associated with their carboxyl group –COOH. Sulfonic acids, containing the group –SO2OH, are relatively stronger acids. Alcohols, with –OH, can act as acids but they are usually very weak. The relative stability of the conjugate base of the acid determines its acidity. Other groups can also confer acidity, usually weakly: the thiol group –SH, the enol group, and the phenol group. In biological systems, organic compounds containing these groups are generally referred to as organic acids.
Using a simple acid mobile phase (typically dilute sulfuric acid), a wide variety of organic acids can be separated without the need of a gradient. By altering the concentration of the acid mobile phase, organic acid separations can be maximized for your particular sample. Benson Polymeric offers two types of cross-linked polymers. The degree of cross-linkage determines the porosity of the polymers which can be used to enhance certain separations. Benson Polymeric also recommends using column ovens in combination with our columns because the best separations are usually obtained at elevated temperatures (typical range 30 – 80 Celsius). By combining eluent strength, temperature and cross-linkage, Benson Polymeric organic acids analysis columns can separate a wide range of samples.
